Bifurcate evolution of quinone synthetases in basidiomycetes

Seibold PS, Lawrinowitz S, Raztsou I, Gressler M, Arndt H-D, Stallforth P, Hoffmeister D

Fungal Biol. Biotechnol. 2023, 10, 14. DOI: 10.1186/s40694-023-00162-1

Natural products from reconstructed bacterial genomes of the Middle and Upper Paleolithic

Klapper M, Hübner A, Ibrahim A, Wasmuth I, Borry M, Haensch VG, Zhang S, Al-Jammal WK, Suma H, Fellows Yates JA, Frangenberg J, Velsko IM, Chowdhury S, Herbst R, Bratovanov EV, Dahse H-M, Horch T, Hertweck C, González Morales MR, Straus LG, Vilotijevic I, Warinner C, Stallforth P

Science 2023, 380, 618. DOI: 10.1126/science.adf5300

Scientist holding an ancient maxillary bone

Source: Werner Siemens Foundation, Felix Wey

Ecological Niche-Inspired Genome Mining Leads to the Discovery of Crop-Protecting Nonribosomal Lipopeptides Featuring a Transient Amino Acid Building Block

Götze S, Vij R, Burow K, Thome N, Urbat L, Schlosser N, Pflanze S, Müller R, Hänsch VG, Schlabach K, Fazlikhani L, Walther G, Dahse HM, Regestein L, Brunke S, Hube B, Hertweck C, Franken P, Stallforth P

J. Am. Chem. Soc. 2023, 145, 4. DOI: 10.1021/jacs.2c11107

Functional Modulation of Chemical Mediators in Microbial Communities

Stallforth P, Hertweck C, Mittag M, Brakhage AA, Hellmich U

Trends Biochem. Sci. 2023, 48, 1. DOI: 10.1016/j.tibs.2022.07.006



Yellow polyketide pigment suppresses premature hatching in social amoeba

Günther M, Reimer C, Herbst R, Kufs JE, Rautschek J, Ueberschaar N, Zhang S, Peschel G, Reimer L, Regestein L, Valiante V, Hillmann F, Stallforth P

Proc. Natl. Acad. Sci. U.S.A. 2022, 119, 43. DOI: 10.1073/pnas.2116122119

Heat map of pks expression and detection of pks5 promoter-driven RFP expression

Tyrosine bioconjugation with hypervalent iodine

Declas N, Maynard JRJ, Menin L, Gasilova N, Götze S, Sprague JL, Stallforth P, Matile S, Waser J. Tyrosine bioconjugation with hypervalent iodine.

Chem. Sci. 2022, 13, 43. DOI: 10.1039/d2sc04558c

Graphical abstract: Tyrosine bioconjugation with hypervalent iodine

The potential of amoeba-based processes for natural product syntheses

Kufs JE, Reimer C, Stallforth P, Hillmann F, Regestein L

Curr. Opin. Biotechnol. 2022, 77. DOI: 10.1016/j.copbio.2022.102766

Approaches for natural product formation with the amoeba Dictyostelium discoideum and subsequent process scale-up

Total Synthesis and Structure Correction of the Cyclic Lipodepsipeptide Orfamide A

Bando Y, Hou Y, Seyfarth L, Probst J, Götze S, Bogacz M, Hellmich UA, Stallforth P, Mittag M, Arndt HD

Chem. Eur. J. 2022, 28, 20. DOI: 10.1002/chem.202104417



Total synthesis and bioactivity mapping of geodiamolide H

Nasufović V, Küllmer F, Bößneck J, Dahse HM, Görls H, Bellstedt P, Stallforth P, Arndt HD

Chem. Eur. J. 2021, 27, 45. DOI: 10.1002/chem.202100989

Total synthesis of geodiamolide H

Lipopeptide-mediated bacterial interaction enables cooperative predator defense

Zhang S, Mukherji R,1, Chowdhury S , Reimer L, Stallforth P

Proc. Natl. Acad. Sci. U.S.A. 2021, 118, 6. DOI: 10.1073/pnas.2013759118

Phylogeny of 58 unique bacterial isolates

The Landscape of Recombination Events That Create Nonribosomal Peptide Diversity

Baunach M, Chowdhury S, Stallforth P, Dittmann E

Mol. Biol. Evol. 202138, 5. DOI: 10.1093/molbev/msab015

Projection of the deduced exchange units on the structure of SrfA–C



Special Issue in Honor of Professor Jon Clardy

Seyedsayamdost M R, Stallforth P

J. Nat. Prod. 2020, 83, 3. DOI: 10.1021/acs.jnatprod.0c00199

Structure elucidation of bacterial nonribosomal lipopeptides

Götze S, Stallforth P

Org. Biomol. Chem. 2020, 18. DOI: 10.1039/C9OB02539A

Analytical techniques and tools to elucidate the structure of bacterial nonribosomal lipopeptides

Chimeric LuxR Transcription Factors Rewire Natural Product Regulation

Mukherji R, Zhang S, Chowdhury S, Stallforth P

Angew. Chem. Int. Ed. 2020, 132, 15. DOI: 10.1002/ange.201914449

Activation of silent bacterial biosynthetic gene clusters by LuxR-type transcriptional activator proteins



Biosynthesis of Pseudomonas‐Derived Butenolides

Klapper M, Schlabach K, Paschold A, Zhang S, Chowdhury S, Menzel K-D, Rosenbaum M A, Stallforth P

Angew. Chem. Int. Ed. 2019, 59, 14.  DOI: 10.1002/anie.201914154

Styrolides representing a structural and biosynthetic link between tetronic acids and γ-butyrolactones

Structure elucidation of the syringafactin lipopeptides provides insight in the evolution of nonribosomal peptide synthetases

Götze S, Arp J, Lackner G, Zhang S, Kries H, Klapper M, Garcia-Altares M, Willing K, Günther M, Stallforth P

Chem. Sci. 2019, 10. DOI: 10.1039/C9SC03633D

Diversification of modular nonribosomal peptide synthetases

Disruption of membrane integrity by the bacteria-derived antifungal jagaricin

Fischer D, Gessner G, Fill TP, Barnett R, Tron K, Dornblut K, Kloss F, Stallforth P, Hube B, Heinemann SH, Hertweck C, Scherlach K, Brunke S

Antimicrob. Agents Chemother 2019, 63, 9. DOI: 10.1128/AAC.00707-19

Susceptibility analysis D. discoideum and killing of C. albicans

Bioactivity and Mode of Action of Bacterial Tetramic Acids

Klapper M, Paschold A, Zhang S, Weigel C, Dahse H-M, Götze S, Pace S, König S, Rao Z, Reimer L, Werz O, Stallforth P

ACS Chem. Biol. 2019, 14, 8. DOI: 10.1021/acschembio.9b00388

Microbially produced 3-acyltetramic acids displaying a diverse range of biological activities



The Chemoattractant Glorin Is Inactivated by Ester Cleavage during Early Multicellular Development of Polysphondylium pallidum

Heinrich D, Barnett R, Tweedy L, Insall R, Stallforth P, Winckler T

ACS Chem. Biol. 2018, 13, 6. DOI: 10.1021/acschembio.8b00046

Inactivation of glorin cuased by extracellular enzymatic activity (glorinase) in the glorin-responsive group 2 taxon Polysphondylium pallidum

Synthesis, Liposomal Formulation, and Immunological Evaluation of a Minimalistic Carbohydrate-α-GalCer Vaccine Candidate

Broecker F, Götze S, Hudon J, Rathwell DCK, Pereira CL, Stallforth P, Chakkumkalag A, Seeberger PH

J. Med. Chem. 2018, 61, 11. DOI: 10.1021/acs.jmedchem.8b00312

Two-component platform based on the facile conjugation of carbohydrate antigens to α-galactosylceramide to yield fully synthetic vaccine candidates

Synergistic activity of co-secreted natural products from amoebae-associated bacteria

Arp J, Götze S, Mukherji R, Mattern D, Garcia-Altares M, Klapper M, Brock D, Brakhage AA, Strassmann J, Queller D, Bardl B, Willing K, Peschel G, Stallforth P

Proc. Natl. Acad. Sci. U.S.A. 2018, 115, 15. DOI: 10.1073/pnas.17217901

Two independent QS systems regulate the production of jessenipeptin and mupirocin, which synergistically inhibit the growth of MRSA

Bacterial Alkaloid Biosynthesis: Structural Diversity via a Minimalistic Nonribosomal Peptide Synthetase

Klapper M, Braga D, Lackner G, Herbst R, Stallforth P

Cell Chem. Biol. 2018, 25, 6. DOI: 10.1016/j.chembiol.2018.02.013

The Role of Bacterial Natural Products in Predator Defense

Klapper M, Arp J, Günther M, Stallforth P

Synlett 2018, 29, 05. DOI: 10.1055/s-0037-1609226

Natural Products from Social Amoebae

Barnett R, Stallforth P

Chem. Eur. J. 2018, 24, 17. DOI: 10.1002/chem.201703694

Social amoebae displaying a very diverse secondary metabolism



Structure, Biosynthesis, and Biological Activity of the Cyclic Lipopeptide Anikasin

Götze S, Herbst-Irmer R, Klapper M, Görls H, Schneider KRA, Barnett R, Burks T, Neu U, Stallforth P

ACS Chem. Biol. 2017, 12, 10. DOI: 10.1021/acschembio.7b00589

Dual functionality of anikasin

Lysinibacillus fusiformis M5 Induces Increased Complexity in Bacillus subtilis 168 Colony Biofilms via Hypoxanthine

Gallegos-Monterrosa R, Kankel S, Götze S, Barnett R, Stallforth P, Kovács AT

J. Bacteriol. 2017, 199, 22. DOI: 10.1128/jb.00204-17

Single-strain and mixed colonies of B. subtilis and selected soil strains

Rationalizing the Right Ratios

Arp J, Stallforth P

Cell Chem. Biol. 2017, 24, 5. DOI: 10.1016/j.chembiol.2017.05.007

Figure thumbnail gr2

Versatile synthesis of the signaling peptide glorin

Barnett R, Raszkowski D, Winckler T, Stallforth P

Beilstein J. Org. Chem. 2017, 13. DOI: 10.3762/bjoc.13.27




Bacterial Alkaloids Prevent Amoebal Predation

Klapper M, Götze S, Barnett R, Willing K, Stallforth P

Angew. Chem. Int. Ed. 2016, 55, 31. DOI: 10.1002/anie.201603312

Description unavailable



Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus

Braesel J, Götze S, Shah F, Heine D, Tauber J, Hertweck C, Tunlid A, Stallforth P, Hoffmeister D

Chem. Biol. 2015, 22, 10. DOI: 10.1016/j.chembiol.2015.08.016

Total Synthesis of Legionaminic Acid as Basis for Serological Studies

Matthies S, Stallforth P, Seeberger PH

J. Am. Chem. Soc. 2015, 137, 8. DOI: 10.1021/jacs.5b00455

Abstract Image



A semisynthetic carbohydrate-lipid vaccine that protects against S. pneumoniae in mice

Cavallari M, Stallforth P, Kalinichenko A, Rathwell DCK, Gronewold TMA, Adibekian A, Mori L, Landmann R, Seeberger PH, DeLibero G

Nat. Chem. Biol. 2014, 10. DOI: 10.1038/nchembio.1650

Atlas for drug discovery

Stallforth P, Clardy J

Proc. Natl. Acad. Sci. U.S.A. 2014, 111, 10. DOI: 10.1073/pnas.140051611

A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene

Stallforth P, Brock DA, Cantley AM, Tian X, Queller DC, Strassmann JE, Clardy J

Proc. Natl. Acad. Sci. U.S.A. 2014, 110, 36. DOI: 10.1073/pnas.1308199110

X-ray crystallography: One size fits most

Stallforth P, Clardy J

Nature 2014, 495. DOI: 10.1038/495456a



Protein Evolution: When Two Become Three

Stallforth P, Clardy J

Curr. Biol. 2012, 22, 17. DOI: 10.1016/j.cub.2012.07.055

Figure thumbnail gr1

De novo chemoenzymatic synthesis of sialic acid

Stallforth P, Matthies S, Adibekian A, Gillingham DG, Hilvert D, Seeberger PH

Chem. Commun. 2012, 48, 98. DOI: 10.1039/C2CC37305J

Graphical abstract: De novo chemoenzymatic synthesis of sialic acid



Comparative bioinformatics analysis of the mammalian and bacterial glycomes

Adibekian A, Stallforth P, Hecht ML, Werz DB, Gagneux P, Seeberger PH

Chem. Sci. 2011, 2. DOI: 10.1039/C0SC00322K

The 10 most abundant monosaccharides found in bacteria, but not in mammals (relative abundance in all bacteria indicated in parentheses).



Recent advances in carbohydrate-based vaccines

Hecht ML, Stallforth P, Silva DV, Adibekian A, Seeberger PH

Curr. Opin. Chem. Biol. 2009, 13, 3. DOI: 10.1016/j.cbpa.2009.05.127

2009 Claude S. Hudson Award in Carbohydrate Chemistry. Carbohydrates: a frontier in medicinal chemistry

Stallforth P, Lepenies B, Adibekian A, Seeberger PH

J. Med. Chem. 2009, 52, 18. DOI: 10.1021/jm900819p